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JDRF nPOD Newsletter for Investigators
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Table of Contents:
Take a look at this ad for organ donation by an OPO in Argentina, the Fundacion Argentina de Transplante Hepatico.

 

Case narratives launch

nPOD has created case narratives from nPOD cases to help investigators better characterize nPOD donors. This information is extracted from hospital charts and medical/social interviews obtained from organ procurement organizations (OPOs). The goal is to provide pertinent diabetes and medical information to help investigators select the best cases for their research projects. Currently, there are about 130 case narratives available in DataShare, most of which are T1D and autoantibody positive cases. More will be made available when they are completed.

Investigators can access this info in DataShare by doing the following:
After logging into DataShare, users will see an option called “nPOD Case Histories and Narratives.”  Click on this and you will be directed to a page that contains the case narratives.  In the upper right hand corner of the page, click on the “Abstracts” link under the case narratives heading. See screen shots below for further detail.

    




Below is an example of a case narrative for case 6054.


 nPOD case 6054: Type 1 Diabetes
            This donor was a 35 year old Caucasian female with a 30 year history of T1D. Her weight was 75kg, height 157.4cm, and BMI 30.3m2. ABO/Rh was A positive, with HLA serotype A*1/-; B*8/2; DR*-/44.  Infectious serology testing was reactive for EBV IgG. Her medical history included a hospitalization for severe hypoglycemia 6 months prior to terminal event, end stage renal disease requiring hemodialysis, hypertension, diabetic neuropathy and retinopathy. Home medications included Humulin R and Humulin N insulins, Coneg, Vasotec, Phoslo, and Norvasc (dosages and frequency not available).  Social history included remote history of cigarette smoking 0.25 pack per day for 1 year and occasional alcohol consumption, 2-3 beers per month for the last 5 years.
            The donor had a syncopal episode followed by a seizure at home. She was taken to a local Emergency Department where she was intubated. Laboratory results included blood glucose 345mg/dL, pH 7.61, and HCO3 23mEq/L. HbA1c was not available. Head CT scan showed a large intraparenchymal hemorrhage centered with the right basal ganglia extending into the ventricular system, subfalcine herniation, and diffuse sulcal effacement. She was transferred to another facility for higher level of care where a repeat CT head showed herniation. Medications given in hospital included dopamine, neosynephrine, levothyroxine, and Cardene.  Brain death was declared 21.5 hours post-admit. Admit to cross clamp time was 45.5 hours.
Post-recovery labs: C-peptide <0.05ng/dL
Autoantibodies (RIA): mIAA 0.404 [<0.010 normal]
HLA genotype: A*01:01, 02:01;  B*08:01, 44:02; C*05:01, 07:01; DRB1*03:01, 04:01; DQA1*03:01, 05:01; DQB1*02:01, 03:02; DPA1*01:03, 02:01; DPB1*01:01, 02:01.

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IHC antibody log resources available 

nPOD is maintaining a log for all IHC antibodies used in human tissues extracted from publications using nPOD samples. This log will be updated with future relevant publications. This valuable resource can now be found on nPOD's Publications and Antibodies page.

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Helmsley Charitable Trust nPOD George Eisenbarth Award for Team Science Pilot funds available for investigators

Please note that funds are available from the Helmsley Charitable Trust for pilot studies to support innovative projects. The updated investigator application form on our website now includes a section where you can specify whether you wish to be considered for this funding opportunity, along with review criteria. Applications can be submitted at any time and we hold reviews approximately every two months. The next review is likely to occur in late August or early September. We look forward to hearing from you.

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Welcome Dr. Sirlene Cechin, nPOD Working Groups/DataShare Scientist

Dear nPOD Investigators:

Please welcome Sirlene R. Cechin, MS, PhD, MBA, to the nPOD team. Sirlene has joined us to help develop the scientific interactions supported by the nPOD George Eisenbarth Award for Team Science awarded by the Helmsley Charitable Trust.  Sirlene will be based with Alberto at the Diabetes Research Institute (DRI), University of Miami.

Sirlene received her PhD from the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, where she worked under the direction of Professor Richard Burnard Rodnight, a renowned scientist in cell signaling and protein phosphorylation. She joined the DRI in 2005, and has since worked on several diabetes research projects. Throughout these years, her research focused on improving islet cell transplantation outcomes as well as identifying molecules with therapeutic potential to cure or to prevent the onset of T1D in experimental models. Her research has spanned from the delivery of cytoprotective genes into human pancreatic islets (Gay-Rubinstein C. et al., Cell Transplantation, 2008, the repurposing of FDA approved drugs (Exenatide/Byetta) as an anti-inflammatory agent in human islets both in vitro and in islet transplanted under the kidney capsule of mice (Cechin S. et al., Cell Transplantation 2012). She also worked with the DRI Pancreatic Development & Stem Cell Laboratory where she took an active role in several projects focused on the generation of insulin producing cells from both induced pluripotent stem cells (IPSCs) and human embryonic stem cells (hESCs) (Cechin S. et al., Stem Cells, 2014). Lastly, Sirlene’s latest efforts were focused on establishing the potential of a Smad7 anti-sense to cure or prevent the onset of T1D using the NOD animal model. Her results have been presented at conferences (ADA 2014 and 2015) while she finalizes a manuscript for publication. These results are very encouraging and hold a clinical translational potential.

Thus, Sirlene is already quite knowledgeable about type 1 diabetes research and can easily work with you to help advance your projects on nPOD specimens and collaborations. She will also be contacting you to help you design formats for depositing your data in DataShare, to facilitate this process.

Please join me in welcoming Sirlene to the nPOD Team.
Alberto


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New nPOD staff


Amanda Myers, Investigator Coordinator
A University of Florida alumna, Amanda has over ten years of experience in both administration and customer service. Prior to joining the nPOD team, she was the Intake Coordinator for the Office of the Public Defender for the Eighth Judicial Circuit.




Maria Peterson, Sr. Biological Scientist
Maria will serve as nPOD’s Sr. Biological Scientist. Maria’s Bachelor Degree is from University of Wisconsin-Madison where she majored in zoology.  She got her Master’s Degree in Molecular and Microbiology from University of North Florida in 2014. Her Master’s work was concentrated on amino acid transport in intestinal membrane of lobster and shrimp. Maria comes to nPOD with wide-range experience in analytical toxicology using GC-MS and LC-MS. She recently moved from Jacksonville, FL to Gainesville and looking forward to become a Gator fan.


Stephen Selman, Senior Lab Technician
Stephen is our new Sr. Lab Tech. Stephen received his Bachelor Degree in Biology from University of Florida in 2013. After working for several months as a Sr. Secretary at Pathology Department of UF, he realized that his passion lies within the fields of biology and medicine. He joined the nPOD team in order to explore T1D research and gain experience before applying to medical school. Stephen is also a dedicated fitness enthusiast.


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 Reminder: no sample passage

Please note that nPOD samples cannot be passed onto other investigators without prior written approval from nPOD's OPPC Director, Irina Kusmartseva. University of Florida requires nPOD to keep track the chain of custody for human samples collected. Your signed user agreement and MTA help ensure this process. Sample distributions are based on your approved nPOD project, and should not be passed onto anyone else. If you requested more nPOD samples than you need, please contact us so we can arrange return of the samples to nPOD.

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nPOD presents in Japan


Dr. Campbell-Thompson participated in the 58th annual Japanese Diabetes Society giving a lecture titled "Histopathology of Insulitis in Type 1 Diabetes: Lessons from the Network for Pancreatic Organ donors with Diabetes" for a symposium on “New insights in human type 1 diabetes” on May 25. The symposium site was the cruise ship Pacific Venus in Shimonoseki, Japan. She then traveled to Tokyo where she was hosted by Dr. Tetsuro Kobayashi, nPOD investigator, for a lecture titled “Pancreas Exocrine and Endocrine Pathology in the Progression to Type 1 Diabetes.” 





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Highlight of recent publications

Mark Huising
Huising, an nPOD investigator, just published a paper in Nature Medicine suggesting peptide hormone urocortin3 (Ucn3) is a key contributor to stable glycemic control and the pathophysiology of diabetes.


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Copyright © 2015 Network for Pancreatic Organ Donors with Diabetes (nPOD), All rights reserved.
|Quarterly Newsletter|

nPOD is a collaborative type 1 diabetes research project funded by JDRF. We support scientific investigators by providing, without cost, rare and difficult to obtain tissues beneficial to their research. nPOD currently supports over 90 type 1 diabetes-related scientific studies at institutions around the world. Our hope is that nPOD will prove a useful resource to the community of researchers dedicated to finding a cure for type 1 diabetes.  For more information, please go to www.jdrfnpod.org